CAR T-Cell (CD19) Enumeration
The CAR T-Cell (CD19) Enumeration Biocytometry Kit utilizes bioparticles displaying the extracellular domain of CD19, enabling precise quantifcation of cells expressing functional anti-CD19 CAR constructs. Identification is not specific to a single anti-CD19 construct, and can be used regardless of CAR T-cell product type. Biocytometry is highly selective, with zero limit of blank and no false positives. The workflow is designed to be user-friendly without the need for expensive instruments or specialized personnel.
Benefits:
- Universal detection of CAR T-cell products (validated on Yescarta and Kymriah products)
- Enumerates only cells with functional CAR constructs
- Higher selectivity than flow cytometry
- Can distinguish between strong and weak binders
- Sensitivity of 10-4
For Reasearch Use Only. To join our Early Access Program, click below.
Working Principle
Biocytometry offers a novel approach for multiparametric immunophenotyping in suspension, employing a bioparticle system engineered to adhere specifically to designated surface markers and activate when adjacent bioparticles are present. Upon activation, these bioparticles begin the production of a reporter molecule. The intensity of the reporter signal is then quantified using a microplate reader, with the strength of the signal directly proportional to the number of cells detected.
Functional CARs (left) are detected by both antibody-based methods and biocytometry. Misfolded CARs (right), however, are not included by biocytometry during detection, accurately identifying only functional CAR T-cells.
Be part of our early access program!
Let us know about a project or need that you have. We’ll be in touch! Please note kits are in limited supply.
